Observations on the species and tissue specificity of histones

Abstract

Recently it has been shown that by a combination of two previous chro- matographic methods (Davison, 1957; Phillips & Johns, 1959) it is possible to resolve calf-thymus histone into three main groups, namely, f 1, a very lysine-rich fraction; f2, a slightly lysine-rich fraction and f 3, an arginine-rich fraction (Johns, Phillips, Simson & Butler, 1960), but starch-gel electrophoresis showed that these were also com- plex (Johns, Phillips, Simson & Butler, 1961). A method of extracting f3 in quantity from calf thymus was given by the same authors.