Uric Acid

Abstract

Binding of sodium urate to human serum albumin (HSA) was measured by continuous ultrafiltration at pH 7.4 and ionic strength 0.16 over the concentration range 1-13 mg/100 ml. The percent sodium urate bound to 5 g/100 ml HSA was constant over this concentration range: 30.3 (SE+/-0.6)% being bound at 4 degrees C, 22.6+/-0.3% at 22.5 degrees C, and 19.6+/-0.3% at 37 degrees C. Derived association constants, assuming one binding site were 6.0 x 10(2) M(-1) (4 degrees C), 4.47 x 10(2) M(-1) (22.5 degrees C), and 3.88 x 10(2) M(-1) (37 degrees C).