Formation of Wound Tissue of Gracilaria chorda Holmes (Gracilariaceae) in Culture

Abstract

The wound healing of GTacilaTia clwrda is described.At the surface of cut tissues, wounded cells lost their cytoplasm, leaving only the cell walls still attached.However, inside cells (i.e.those next to wounded cells) remained intact, and the nuclei and cytoplasm of some of these cells moved toward the wound within 24 h after the wounding.In these cells, Golgi bodies increased in number and then produced many secretory vesicles.Simultaneously, many multivesicular bodies appeared in these cells and released their contents into the cell wall.Two days after wounding, the first cytokineses occurred in the inside cells, forming new cells along the cut surface.The cell walls between the wounded and new inside cells became thicker and separated the pit plugs connected to wounded cells from the inside cells.The new cells divided further to form wound tissue with up to 3 layers, which eventually developed into cortex-like tissue.