Methods for the estimation of pyrazinamide and pyrazinoic acid in body fluids.

Abstract

Methods for the estimation of pyrazinamide in serum and of pyrazinamide and pyrazinoic acid in urine using an anion-exchange resin (Dowex-1) have been described. Recoveries were quantitative, and the sensitivity was 5 μg/ml for all 3 estimations. In serum, pyrazinoic acid at a concentration of 50 μg/ml did not interfere with the estimation of pyrazinamide. In urine, pyrazinamide and pyrazinoic acid could be separated from each other and estimated even when the 2 compounds were present together at concentrations of 2000 μg/ml each.