Hemoglobin Synthesis in Avian Erythrocytes

Abstract

Preparation of C&s--Heparinized(1 mg/lO ml) whole blood from adult pigeons was centrifuged at 2500 r.p.m. (1500 X g) at O-4" for 10 minutes and the plasma and huffy coat were removed.The red cells were washed in 3 to 5 volumes of cold 0.9% sodium chloride solution three times.The third time the top 5% of the red cell layer was discarded to insure complete removal of white