Identification of Plasmodium Falciparum-Infected Mosquitoes by a Double Antibody Enzyme-Linked Immunosorbent Assay

Abstract

A double antibody micro enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium falciparum sporozoites in mosquitoes is described. Using monoclonal antibodies made against South American P. falciparum sporozoites, the ELISA was able to detect and identify sporozoite antigens of South American and Asian origins in extracts of dried infected mosquitoes.