Methods in molecular biology · 2011 · 20 citations · 6 references
Life SciencesBiochemistryMolecular BiologyCell SignalingMolecular Cell BiologySignal TransductionCell Signaling BiophysicsCell Signaling NetworksCellular ImagingCellular VisualizationProtein DynamicsProtein Kinase CascadesFret ImagingKinase Activation MechanismsKinase Activity ProfilingKinase ImagingProtein Phosphorylation DynamicsSubcellular Dynamics ImagingSubcellular Protein Dynamics
The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.
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Insulin disrupts β-adrenergic signalling to protein kinase A in adipocytes
Jin Zhang, Christopher J. Hupfeld, Susan S. Taylor et al. · Nature · 2005 · 315 citations