Systematic distribution of muscle fibre types in the rat and rabbit diaphragm: a morphometric and ultrastructural analysis.

Abstract

The histochemical and ultrastructural characteristics of the adult rat and rabbit costal diaphragm were investigated. On the basis of enzyme histochemistry, the rat diaphragm was found to contain 42% and 39% Type I, 24% and 25% Type II A and 33% and 34% Type II B fibres on the thoracic and abdominal surfaces respectively. The rabbit costal diaphragm contained 18% and 26% Type I, 46% and 39% Type II A and 35% and 34% Type II B fibres on the thoracic and abdominal surfaces respectively. Differences in the proportion of each muscle fibre type were also observed between diaphragmatic regions (ventral, medial and dorsal) in the rat as well as in the rabbit. Differences in muscle architecture were also noted on the basis of stereological analysis in estimation of volume density, surface density, numerical density and cross-sectional areas of each muscle fibre type. The fine structural analysis of all three fibre types also showed significant differences in the width of the A-bands and Z-lines between the muscle fibre types of the rat and rabbit costal diaphragm.