Humana Press eBooks · 2003 · 302 citations · 0 references
AgriculturePlant BiologyPlant SciencesPlant BiotechnologyPlant GeneticsPlant VirologyPlant Gene ExpressionPlant VirusesVirus-host InteractionsVirus-induced Gene SilencingCrop Improvement ApplicationsViral Gene FunctionVirus-induced Gene Silencing Vectors
In the postgenomic era, large-scale functional genomic approaches are necessary for converting sequence information into functional information. A para-genetic approach, called virus-induced gene silencing (VIGS), offers a rapid means of gaining insight into gene function in plants. VIGS system could be used to suppress endogenous gene expression by infecting plants with a recombinant virus vector (VIGS vector) carrying host-derived sequence. Here, we describe the use of tobacco rattle virus (TRV)-based VIGS technique to study gene function in Nicotiana benthamiana and tomato.