Comparative lectin-binding patterns in the epidermis and dermal glands of Bufo bufo (L.) and Xenopus laevis (Daudin).

Abstract

Bouin-fixed and paraffin-embedded sections from the dorsal skin of Bufo bufo and Xenopus Laevis were subjected to lectin histochemistry. A panel of biotinylated lectins (Con-A, PSA, LCA, UEA-I, DBA, SBA, SJA, RCA-I, BSL-I, WGA, s-WGA, PHA-E and PHA-L) and an avidin-biotin-peroxidase complex (ABC) showed a species-specific compartmentalization of saccharides to certain parts of the epidermis and glandular domains. Some marked histochemical differences between the two examined species adapted to fully aquatic (X. laevis) or semiterrestrial (B. bufo) environments may be relevant of a relationship existing between habitat selection and the glycosaminoglycans content of the skin. In addition the technique used in this paper may be applicable for further studies of the carbohydrate composition in various tissues of lower vertebrates.