PubMed · 1990 · 80 citations · 0 references
Life SciencesInfectious DiseasesMicrobiologyMolecular VirologyVaccinologyVirologyAnimal Cell CultureAnimal Infectious DiseasesAnimal VirusesVeterinary VirologyViral InfectionsViral VaccinesCell Culture-based Vaccine ProductionExperimental Animal Models Of Viral InfectionInoculationViral CultivationViral CultureViral Immunity In SwineViral Pathogenesis In SwineVirus Production
The Chinese strain of hog cholera virus (HCV) was adapted to suspension cultures of the established swine kidney cell line SK6. The strain designated "Cedipest", is produced on the basis of a seedlot system. The masterseed virus was identified in vitro and in vivo, and was found free from extraneous pig pathogenic viruses by repeated animal inoculation followed by appropriate serological tests. A distinct and reproducible relationship was ascertained between infectivity in vitro and protection. Pigs inoculated with 400-600 TCID50 of the Cedipest strain proved fully protected against challenge with greater than 100 pig LD50 of a virulent strain of HCV at 7 days and at 6 month post vaccination.