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A rapid optimized protocol for downward alkaline Southern blotting of DNA.
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1993
Year
ChromatinDna SequencingDownward DirectionMolecular Biological MethodNatural SciencesGeneticsConventional Upward-transferred BlotsDna AnalysisDna ReplicationMolecular BiologyNucleic Acid Amplification TestMolecular GeneticsNucleic Acid AmplificationDna ComputingMedicineGenome EditingBiophysicsM Naoh
A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.