Effect of different media and growth regulators on in vitro shoot proliferation of olive cultivar 'Moraiolo'.

Abstract

The effect of Woody Plant Medium (WPM) and Olive Medium (OM) with various concentrations of Zeatin & Benzylaminopurine (BAP) solely or in combinations with each other, was investigated on In vitro shoot proliferation of the olive cultivar ‘Moraiolo’. Olive medium proved to be the most effective one, resulting in better and morphologically superior microshoots as compared to woody plant medium. Zeatin (3.0 mg l -1 ) in combination with 0.5 mg l -1 BAP resulted in highest number of microshoots per explant (0.84), with 2.25 cm shoot length and 1.88 number of nodes on olive medium as compared to its single use. The best interaction of both media with cytokinins occurred when 3.0 mg l -1 Zeatin was used in combination with 0.5 mg l -1 BAP.