Spectrophotometric determination of lamivudine and stavudine

Abstract

A simple, sensitive spectrophotometric method has been developed for the determination of lamivudine and stavudine In pure and its pharmaceutical formulations. Lamivudine and stavudine give green coloured chromogens with 3-methyl-2-benzothiozolinone hydrazone hydrocloride and ferric chloride with absorption maximum at 660 nm and 630 nm, respectively. The chromogens obey Beer's law in the concentration ranges of 2.5 to 40 I¼g/ml and 2.5 to 12.5 I¼g/ml, respectively for lamivudine and stavudine.