As an essential plant micronutrient, copper (Cu) is required as a component of several enzymes, but it can be highly toxic to plants when present in excess quantities. Nitrogen (N) application can help to alleviate the phytotoxic effects of heavy metals, including Cu, and different N forms significantly affect the uptake and accumulation of heavy metals in plants. The aim of this study was to determine the effects of different N forms, i.e., ammonium (NH₄⁺) and nitrate (NO₃^-), on Cu detoxification in wheat seedlings. The inhibition of seedling growth under excess Cu was more obvious in wheat plants supplied with NO₃^- than in those supplied with NH₄⁺. This growth inhibition was directly induced by excess Cu accumulation and reduced absorption of other mineral nutrients by the plants. Compared with seedlings treated with NO₃^-, those treated with NH₄⁺ showed a decrease in Cu-induced toxicity as a result of increased antioxidant capacity in the leaves and a lower redox potential in the rhizosphere. Furthermore, treatment with NH₄⁺ decreased the loss of mineral nutrients in wheat seedlings exposed to excess Cu. In conclusion, compared with supplying NO₃^-, supplying NH₄⁺ to wheat seedlings under Cu stress improved their ability to maintain their nutritional and redox balance and increased their antioxidant capacity, thereby preventing a decline in photosynthesis. According to our results, NH₄⁺ is more effective than NO₃^- in reducing Cu phytotoxicity in wheat seedlings.

#	Concept	H-Index	Publications	Citations
1	Medicine	2	2	60
2	Cell Biology	2	2	60
3	Natural Sciences	1	1	17
4	Pathology	1	1	43
5	Pharmacology	1	1	43

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