A novel actinobacterium, strain YIM 96748^T, was isolated from a saline soil sample collected from the south bank of Aiding Lake in Xinjiang Province, Northwest China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 96748^T is closely related to Amycolatopsis cihanbeyliensis BNT52^T (98.9%) and Amycolatopsis jiangsuensis KLBMP 1262^T (97.2%). The DNA-DNA relatedness between strain YIM 96748^T and its closest type strain A. cihanbeyliensis BNT52^T was 59.6%. The average nucleotide identity between strain YIM 96748^T and its neighbor strain was 88.97%. Based on the genotypic and phenotypic characteristics, it is concluded that strain YIM 96748^T represents a novel species of the genus Amycolatopsis, whose name was proposed as Amycolatopsis aidingensis sp. nov. The type strain is YIM 96748^T. To investigate the biosynthetic potential of producing secondary metabolites, the complete genome of YIM 96748^T was sequenced and analyzed. The complete genome sequence of YIM 96748^T consists of a 7,657,695-bp circular chromosome, comprising 7,162 predicted genes with a DNA G + C content of 70.21 mol%. Fifty-one putative biosynthetic gene clusters of secondary metabolites were found, including the antibacterial/antitumor agent TLN-05220, the antibacterial agent nocardicin A, the antifungal agent nystatin A1, and the osmolyte ectoine. The investigation of the secondary metabolites of A. aidingensis YIM96748^T led to the discovery of two new phenylpropyl acetate enantiomers, amycoletates A (1) and B (2), and five known compounds: 4-hydroxy phenethyl acetate (3), 2-p-acetoxyphenylethanol (4), (S)-ethyl indole-3-lactate (5), (R)-ethyl indole-3-lactate (6), and p-hydroxybenzoic acid (7). One of the gene clusters 14, 36, and 43, which contain a single module of polyketide synthase, might be responsible for the biosynthesis of compounds 1 and 2 from compound 7 as a precursor. Further studies, including the one strain many compounds approach (OSMAC) and genetic modification, are needed to explore novel compounds from this talented halophilic Amycolatopsis strain.

#	Concept	H-Index	Publications	Citations
1	Medicine	1	1	24
2	Biology	1	1	24
3	Microbiology	1	1	24
4	Bacteriology	1	1	24
5	Microbial Ecology	1	1	24

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