21:I[432972,["/_next/static/chunks/bb923814e1f4d2e9.js","/_next/static/chunks/dd3ee9f5b7716ca6.js","/_next/static/chunks/4204a0ae82542ca8.js","/_next/static/chunks/1270dead247da766.js","/_next/static/chunks/6e1aa89a642f40be.js","/_next/static/chunks/2d7aa753e81324a8.js","/_next/static/chunks/8ffd0b71567920d8.js","/_next/static/chunks/67ea6dfd21f16c5f.js","/_next/static/chunks/24eb4379f68013fd.js","/_next/static/chunks/b8cd775ffdf935bd.js","/_next/static/chunks/983103cd9eb7b428.js","/_next/static/chunks/263f2cdcb9aa2341.js","/_next/static/chunks/294b9525248c8e5f.js"],"default"] 22:T4f9,Skin, as the major organ of a human body, is constantly exposed to PM_2.5 stimulation, which may exert specific toxic influences on the physiology of skin. This study aims to investigate the effect of PM_2.5 on the formation of inflammasomes in skin cells and to explore the potential mechanism linking PM_2.5 and skin inflammation. Changes in mRNA and protein levels of inflammasome-related genes were detected by real-time PCR and western blot in human immortalized epidermal cells (HaCaT) treated with PM_2.5 at multiple concentrations for 24 hours. The expression of NLRP1 was increased significantly both in mRNA and protein levels after PM_2.5 exposure while the elevated secretory protein level of IL-1β in cell culture was detected by ELISA, which is one of the main downstream factors of NLRP1. In addition, the upregulation of NLRP1 and IL-1β could be reversed by NF-κB inhibitor indicating that PM_2.5 may promote NLRP1 expression through activating NF-κB pathway. Furthermore, high ROS level was also found in cells treated with PM_2.5 and inhibition of ROS could also reverse NK-κB production stimulated by PM_2.5 that means ROS is involved in this skin inflammation process.

#	Concept	H-Index	Publications	Citations
1	Medicine	6	6	289
2	Cell Biology	3	3	147
3	Engineering	1	1	37
4	Social Sciences	1	1	75
5	Biomedical Engineering	1	1	37

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