The nucleus accumbens core (NAcc) has been repeatedly demonstrated to be a key component of the circuitry associated with excessive ethanol consumption. Previous studies have illustrated that in a nonhuman primate (NHP) model of chronic ethanol consumption, there is significant epigenetic remodeling of the NAcc. In the current study, RNA-Seq was used to examine genome-wide gene expression in eight each of control, low/binge (LD*), and high/very high (HD*) rhesus macaque drinkers. Using an FDR < 0.05, zero genes were significantly differentially expressed (DE) between LD* and controls, six genes between HD* and LD*, and 734 genes between HD* and controls. Focusing on HD* versus control DE genes, the upregulated genes (N = 366) were enriched in genes with annotations associated with signal recognition particle (SRP)-dependent co-translational protein targeting to membrane (FDR < 3 × 10^-59 ), structural constituent of ribosome (FDR < 3 × 10^-47 ), and ribosomal subunit (FDR < 5 × 10^-48 ). Downregulated genes (N = 363) were enriched in annotations associated with behavior (FDR < 2 × 10^-4 ), membrane organization (FDR < 1 × 10^-4 ), inorganic cation transmembrane transporter activity (FDR < 2 × 10^-3 ), synapse part (FDR < 4 × 10^-10 ), glutamatergic synapse (FDR < 1 × 10^-6 ), and GABAergic synapse (FDR < 6 × 10^-4 ). Ingenuity Pathway Analysis (IPA) revealed that EIF2 signaling and mTOR pathways were significantly upregulated in HD* animals (FDR < 3 × 10^-33 and <2 × 10^-16 , respectively). Overall, the data supported our working hypothesis; excessive consumption would be associated with transcriptional differences in GABA/glutamate-related genes.

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