Yuya Toyota

Cyclization of Single-Chain Fv Antibodies Markedly Suppressed Their Characteristic Aggregation Mediated by Inter-Chain VH-VL Interactions

Soichiro Yamauchi, Yoshihiro Kobashigawa, Natsuki Fukuda et al. · Molecules · 2019 · 30 citations · Full text

Pharmacy, Biomolecular Engineering, Molecular Biology +13

Preparation of single-chain Fv antibodies in the cytoplasm of Escherichia coli by simplified and systematic chaperone optimization

Chenjiang Liu, Yoshihiro Kobashigawa, Soichiro Yamauchi et al. · The Journal of Biochemistry · 2019 · 14 citations

Life Sciences, Biochemistry, Biotechnology +17

Molecular Dynamics-Based Design and Biophysical Evaluation of Thermostable Single-Chain Fv Antibody Mutants Derived from Pharmaceutical Antibodies

Kyo Okazaki, Yoshihiro Kobashigawa, Hikari Morita et al. · ACS Omega · 2023 · 13 citations · Full text

Pharmacy, Biophysics, Molecular Biology +17

Expression, Purification and Characterization of CAR/NCOA-1 Tethered Protein in <i>E. coli</i> Using Maltose-Binding Protein Fusion Tag and Gelatinized Corn Starch

Yoshihiro Kobashigawa, Mana Namikawa, Mitsuhiro Sekiguchi et al. · Biological and Pharmaceutical Bulletin · 2020 · 11 citations · Full text

Medicine, Biochemistry, Pharmacology +17